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KMID : 0360220110520010086
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Journal of the Korean Ophthalmological Society
2011 Volume.52 No. 1 p.86 ~ p.92
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The Study of Characteristics of Acellular Porcine Cornea Using Freezing-Thawing-Centrifugation
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Lee Seok-Hyun
Chun Yeoun-Sook
Kim Jae-Chan
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Abstract
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Purpose: To develop a new decellularization technique of porcine cornea using freezing-thawing-centrifugation (FTC) and to examine the characteristics of acellular porcine cornea (APC) for xenograft material.
Methods: Two-hundred micrometer thickness porcine corneas were decellularized with DNase/RNase, followed by 3 freezing-thawing-centrifugations (FTC, group 1), lyophilized FTC-APC (group 2), and chemical enzyme treated APC (CE-APC, group 3). Histologic evaluation to examine cells and collagen matrix, comparison of transparency, and cultivation to determine the viability of stromal cells was performed in fresh porcine cornea and 3 experimental groups.
Results: Decellularization occurred successfully in all experimental groups. Decellularization was confirmed by H&E staining and cultivation. Transparency of group 1 was similar to the normal porcine cornea but transparency of group 2 and group 3 was decreased. Collagen fibers of CE-APC (group 3) were not as well arrayed as FTC-APC (group 2).
Conslusions: Acellularity of porcine cornea was successfully achieved by the FTC method with preservation of the cornea stroma. Novel decellularized porcine cornea can be considered as xenogeneic material for corneal transplantation.
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KEYWORD
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Centrifuge, Decellularization, Porcine cornea, Xenograft
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